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Batches of 5 islets were then incubated for 1 hour at 37 °C in 24-well plates with different glucose concentrations and chemical compounds such as, exendin-(9–39) (Ex9) (Tocris Bioscience, #2081), proglumide (Tocris Bioscience, #1478), sulfated CCK-8 peptide (Cayman Chemical, #23371), and sulfated (pGlu-Gln)-CCK-8 (American Peptide)